Abstract
To determine the prevalence of wheat sensitivity in a randomly selected Australian population, a study was carried out on sera samples of the Geelong Osteoporosis Study (GOS) age-stratified cohorts of men and women who were randomly selected from electoral rolls for the Barwon Statistical Division (n = 1145). The human sera were analysed by ELISA-based method using the ImmunoCAP 100 instrument (Phadia, Sweden). IgE from human sera were bound to the wheat (f4) and milk (f2) allergens that have been previously coated on the ImmunoCAPs. The number of IgE wheat and milk RAST positive individuals was determined. In order to relate the increased IgE immuno-reactivity to allergen symptoms, a questionnaire was established and sent to the blood donors and 974 individuals responded. Of these (n = 974) 147 individuals (15.1%) reported symptoms caused by wheat consumption, 179 (15.6%) and 112 (9.8%) sera showed RAST positive results (IgE > 60 response units) in wheat and milk RAST tests, respectively. However, only 2...
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