Abstract
Studies have shown that various microorganisms, including Pseudomonas aeruginosa, Aspergillus niger, Penicillium notatum , and Monilia albicans are capable of growing in solutions and dispersions of nonionic surfactants of the fatty acid ester type. It was observed that these organisms are capable of splitting the ester linkages of these surfactants. This is first evidenced visually by the appearance of free fatty acids in the inoculated surfactant solutions. Further study revealed that these test organisms produce esterases which split the fatty acid from the surfactant molecule. The presence of esterases was demonstrated using a tributyrin testing procedure. The liberation of free fatty acid from polyoxyethylene 20 sorbitan monostearate (Tween® 60) solutions is reported.
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