The photoreceptors and pigment epithelim of the adult Xenopus retina: morphology and outer segment renewal.

Abstract

Outer segment renewal and the fine structure of photoreceptors and pigment epithelium (p. e.) were studied in the adult Xenopus retina by light microscopic autoradiography and electron microscopy. Following the injection of [ 3 H]leucine, the pattern of labelling observed in receptor outer segments was typical of that reported in other adult retinae: only diffuse labelling was found in cones, but in rods a discrete band of label accumulated at the base of the outer segment and migrated sclerally with time. The rate of band displacement and thus disk addition in Xenopus rods was 1.86 μm/day (or 78 disks/day), which is more than twice that reported for red rods in Rana under similar experimental conditions, although these species have similar metabolic rates. Average rod outer segment (r. o. s.) length did not change, demonstrating a balance between disk addition and shedding. R. o. s. renewal time was about 24 days, corresponding to the time when labelled phagosomes were first found in the p. e. Ultrastructurally, one kind of (red) rod and one kind of cone were found whose outer segments differed in membrane topology. Although microfilaments were found in the apical processes of the p. e. and its cytoplasm contained both pigment granules and myeloid bodies, pigment granules did not migrate into these processes during light adaptation. In addition to possible morphological evidence for phago­somes of cone origin, both large and small rod phagosomes were observed in the p. e. The latter appear to represent small stacks of partial disks shed from individual r. o. s. scallops.