Abstract
Penicillin G acylase from E. coli was used to cleave the phenylacetyl group—the first enzyme‐labile protecting group for the amino function of nucleobases. Now protected oligonucleotides can be unmasked both in solution and on solid supports with this versatile enyzme under mild conditions (pH 7, room temperature). These results may lead to the development of new enzymatic reactions in oligonucleotide and solid‐phase chemistry as well as in combinatorial chemistry.
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