Abstract
I have analysed the amino acid substitution pattern between two pairs of nonbarophilic–barophilic organisms in order to confirm previous results. Indeed, the pattern deriving from a different pair of such organisms led to establish that the origin of the genetic code might have occurred in the ocean abysses. The hydrostatic pressure asymmetry indices computable from these matrices of amino acid substitutions confirm the correlation previously observed, even when differences in GC content are accounted for. As the three substitution matrices are independent between them, the old conclusion that the genetic code originated in the ocean abysses is considerably strengthened.
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