The operating performance of Modified Basalt Fibers (MBF) bio-nest reactor exposed to nano-plastics

Abstract

Biological nests made of Modified Basalt Fiber (MBF bio-nests) serve as effective carriers for enhancing wastewater treatment. However, little is known about their performance when exposed to nano-plastics. This study investigates the decontamination efficiency and microbial functionality of four types of MBF and traditional Basalt Fibers (BF) as carriers in contact oxidation reactors. Compared to BF, MBF demonstrated superior growth effects and biocompatibility within the bio-nest. Ca-MBF and Mn-MBF bio-nests exhibited the highest and most uniform absorption capacities, respectively, alongside increased secretion of total Extracellular Polymeric Substances (EPS) and higher Protein to Sugar (PN/PS) ratios. In sewage environments, all MBF groups displayed stable performance in removing NH4+-N and COD. Significant removal of TN and TP was notably observed in Mn-MBF treatments. Mn and Ca treatments predominantly influenced the Proteobacteria and Bacteroidetes phyla, crucial for nitrogen and phosphorus removal. Following exposure to nano-plastics, Mn-MBF and Ca-MBF treatments maintained high decontamination efficiency, particularly for TP and COD (48.64 % to 57.78 % and 90.91 % to 92.89 %, respectively). The significant removal of NH4+-N and TP only occurred in Mn-MBF and Ca-MBF treatments, which stimulated the growth of bacteria resistant to nano-plastics. Key genera such as Zoogloea and Meganema were identified as dominant, contributing to organic matter decomposition, EPS secretion, biofilm condensation, and enhanced microbial attachment. The findings underscore the structural stability enhancement of Mn-MBF and Ca-MBF bio-nests in contact oxidation reactors, demonstrating their resilience against nano-plastic pollution.