Abstract
ABSTRACTWe developed a convenient, efficient and reliable PCR-based technique to distinguish the frost tolerant and sensitive genotypes in barley. Based on artificial freeze test, significant differences among genotypes were found. In these plants, frost tolerance, expressed as LT50, was strongly correlated with the existence of cold-induced genes. Comparison of LT50 values and the PCR-detected pattern of cold-induced genes showed that Hudson, Dictoo and Krusevac occupied three first positions in terms of a high frost tolerance. After 35 and 49 days of cold acclimation, the Nortstar was found to be the most tolerant wheat genotype (LT50: -16°C). Low correlation between LT50 and existence of cold-induced genes was found for rye and wheat genotypes. Diploid forms of rye plants were found to be more frost tolerant than tetraploid forms.
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