Abstract
Methods nCor a 8 was purified from raw hazelnuts by precipitation and chromatographical steps. Subsequently, the protein was identified by N-terminal sequencing and mass spectrometry. Stability of the protein was investigated by simulated gastric and duodenal digestion assays. Hazelnut lipids were extracted employing the hexane method. Finally, IgE binding activity of native and reduced/alkylated nCor a 8 was tested in ELISA using sera from 6 hazelnut allergic patients.
Highlights
Background and aimHazelnuts are potent inducers of food allergic symptoms, ranging from mild up to severe reactions in sensitized individuals
The protein was identified by N-terminal sequencing and mass spectrometry
MALDI TOF mass spectrometry provided 9.475 kDa corresponding to the theoretical mass of 9.468 kDa
Summary
Background and aimHazelnuts are potent inducers of food allergic symptoms, ranging from mild up to severe reactions in sensitized individuals. The non-specific lipid transfer protein from hazelnut, Cor a 8, a relevant food allergen Pawel Dubiela1*, Sabine Pfeifer1, Merima Bublin1,2, Cristine Hafner1, Karin Hoffmann-Sommergruber1 From Food Allergy and Anaphylaxis Meeting 2014 Dublin, Ireland.
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