Abstract
Twenty-four-hr renal preservation at hypothermic temperatures with pulsatile flow using either Medium 199 or a cryoprecipitate of plasma maintained a patent microcirculation prior to reestablishing blood flow. However, failed reflow of blood, the no-reflow phenomenon, occurred in kidneys preserved with Medium 199 within 4 hr after reimplantation into the host animal, as determined by the intra-arterial injection of carbon. The site of vascular obstruction in the renal microcirculation was documented to be within the glomerulus or in the preglomerular afferent arteriole. Renal preservation using a cryoprecipitate of plasma maintained a patent intrarenal vasculature after restoration of blood flow providing normal or near normal function.
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