Abstract
Objective. To examine the effectiveness of suspensions of embryonic stem cells in experimentally induced aminoglycoside ototoxicity in Guinea pigs.Materials and methods. The study involved 35 adult Guinea pigs. The hearing loss was caused by administration of gentamicin 100 mg per KBW. Intratympanal suspension of 2 million neuronal steam cells in 0.5 mg and sub occipital suspension of 2 million cells in 0.5 mg were administered on the 1st and 15th day.Results. The methodology of using and preparation of the suspension from neuronal stem cells enables to provide their necessary number for transplantation in vivo, remaining the cell characteristics.Aminoglycoside ototoxity caused by a 14-day administration of gentamicin to Guinea pigs in a dose of 100 mg/KBW was accompanied by general intoxication: reduced appetite, weight loss, frequent urination, hair falling out. The administration of neuronal stem cells on the first day, before and after aminoglycoside administration, completely, and on 15 partially neutralizes the symptoms.Fourteen-day administration of gentamicin to Guinea pigs causes a violation of the architectonics and morphology of inner ear microcirculation in psalterial cords.NSC administration on the first day of simulated aminoglycoside ototoxicity prevented its development if 14-day injections of gentamycin, that is confirmed by ABR and morphologically.Conclusions. The conducted experiments show the feasibility of the chosen direction to explore the potential use of neuronal stem cells for the prevention of aminoglycoside ototoxic effects in Guinea pigs.
Highlights
The methodology of using and preparation of the suspension from neuronal stem cells enables to provide their necessary number for transplantation in vivo, remaining the cell characteristics
Aminoglycoside ototoxity caused by a 14-day administration of gentamicin to Guinea pigs in a dose of 100 mg/KBW was accompanied by general intoxication: reduced appetite, weight loss, frequent urination, hair falling out
Fourteen-day administration of gentamicin to Guinea pigs causes a violation of the architectonics and morphology of inner ear microcirculation in psalterial cords
Summary
Сенсоневральну приглухуватість моделювали шляхом введення аміноглікозидного антибіотика гентаміцину сульфату в дозі 100 мг/кг протягом 14 діб. Суспензію нейральних стовбурових клітин вводили в об’ємі 2 млн. Клітин в 0,5 мл – субокципітально у 1-шу та на 15-ту добу експерименту. Введення ЕН в першу добу після моделювання ототоксикозу попереджало його прогресування при 14-денних ін’єкціях гентаміцину, що підтверджено даними функціональних та морфологічних досліджень.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
