Abstract
Objective To define the etiology and genetics of the pathogen causing an acute gastroenteritis outbreak in a college of Shenyang. Methods A total of 15 anal swab samples were collected from students or kitchen staffs of the college where outbroke the epidemic of acute gastroenteritis in December 2015 in Shenyang, Liaoning province. Real-time PCR was performed to identify the infection of Norovirus(NoV). The NoV genes were amplified and sequenced for those positive samples, followed to perform phylogenetic analysis. Results Of the 15 specimens, 12 were NoV positive by Real-time PCR, phylogenetic analysis showed that the infected virus of the outbreak was belonged to NoV genotype GII.17. The homology between the present virus and new mutated GII.17 strain of KR020503 identified in 2014—2015 epidemic outbreak in China was over 99%. Conclusions The new mutated NoV GII.17 caused the acute gastroenteritis outbreak in a college of Shenyang in 2015, NoV GII.17 was detected firstly in Northeast China. Key words: Norovirus; Acute gastroenteritis; Outbreak; GII.17 genotype
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