Abstract
The gene family with sequence similarity 13 member A (FAM13A) has recently been identified as a marker gene in insulin sensitivity and lipolysis. In this study, we first analyzed the expression patterns of this gene in different tissues of adult cattle and then constructed a phylogenetic tree based on the FAM13A amino acid sequence. This showed that subcutaneous adipose tissue had the highest expression in all tissues except lung tissue. Then we summarized the gene structure. The promoter region sequence of the gene was successfully amplified, and the −241/+54 region has been identified as the core promoter region. The core promoter region was determined by the unidirectional deletion of the 5’ flanking promoter region of the FAM13A gene. Based on the bioinformatics analysis, we examined the dual luciferase activity of the vector constructed by the mutation site, and the transcription factors ACSL1 and ASCL2 were found as transcriptional regulators of FAM13A. Moreover, electrophoretic mobility shift assay (EMSA) further validated the regulatory role of ACSL1 and ASCL2 in the regulation of FAM13A. ACSL1 and ASCL2 were finally identified as activating transcription factors. Our results provide a basis for the function of the FAM13A gene in bovine adipocytes in order to improve the deposition of fat deposition in beef cattle muscle.
Highlights
With the improvement in living standards, high-end beef production has attracted more and more attention
We found that family with sequence similarity 13 member A (FAM13A) was expressed most in adipose tissues, except lung tissue (Figure 1A)
We summarized the gene structure of FAM13A and found that the gene is composed of 18 exons in the genome, and the length of the gene is approximately 57 kb
Summary
With the improvement in living standards, high-end beef production has attracted more and more attention. It is important to explore the genes or pathways that control adipocyte differentiation This process, known as adipogenesis, is tightly regulated by a coordinated cascade of transcription factors interacting with each other to regulate the expression of downstream genes involved in adiposity and fat metabolism [6,7,8,9]. Transcription factors, e.g., PPARγ, are protein molecules that can bind to specific sequences upstream of the 50 end of a gene, ensuring that target genes are expressed in a specific time and space.
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