The Mechanism Of Fam134b Mediated Endoplasmic Reticulum Autophagy In Skeletal Muscle After Heavy Load Exercise

Abstract

Endoplasmic reticulum(ER) is a significant organelle involved in the contraction of skeletal muscle. PURPOSE: To investigate the role of FAM134B in ER autophagy of skeletal muscle cells after exercise. METHODS: (1)Subjects and groups:48 healthy male 8-week-old SD rats were randomly divided into control group (Group C, n=8) and exercise group (Group E, n=40). The time items of group E were divided into 5 subgroups of 0h/12h/24h/48h/72h. (2)Exercise program: the rats were trained by using the electric running platform of small animals with large load exercise and reference to the Armstone’s.(3)Sampling and index detection: rats were anesthetized after weighing, soleus was isolated and prepared protein samples and frozen sections;Western blot was used to detect the expression of CRT, FAM134B and LC3 protein in skeletal muscle,and double immunofluorescence staining combined with laser confocal was used to observe the co-location of FAM134B, LC3 and CRT. RESULTS: (1)Compared with group C, the change rate of skeletal muscle protein expression in rats showed that ① CRT (2.080 ± 0.096** 1.325 ± 0.105 * 00 1.395 ± 0.142 * 00 1.315 ± 0.096 * 00.990 ± 0.05200123): in Group E increased by 1.08 times at 0 h after exercise, showing significant difference ② FAM134B (1.301 ± 0.076 * 1.967 ± 0.119** 00 1.379 ± 0.112 * 11.500 ± 0.073 ** 1.277 ± 0.09611): in Group E, it was significantly increased at 12 h and 48 h after exercise, 96.7% and 50% respectively. ③ LC3(1.376 ± 0.132 * 1.799 ± 0.063** 0.562 ± 0.063** 1.659 ± 0.084 ** 1.300 ± 0.067 *1): it was significantly increased in Group E from 0 h to 72 h after exercise. (2) the results of CO location of fam134b and CRT, and co-location of LC3 and CRT showed that compared with group C, group E significantly increased from 0 h to 48 h after exercise. CONCLUSIONS: The expression of FAM134B and LC3 in ER increased and co-located with CRT after heavy load exercise, indicating that FAM134B may be involved in the occurrence of ER autophagy after heavy load exercise.