Abstract
A method for the preparation of microscopic spheres of polymerized immunoglobulins is described. Solid spherical particles were produced employing surfactants to dynamically disperse proteins in mineral oil and subsequently polymerize them with glutaraldehyde. Reproducibility was dependent on several parameters, which had to be kept within narrow limits. Such parameters included mode of dispersing protein in oil, relative as well as absolute amounts of constituents, and pH of the reaction. Immunological activity of the particles, as revealed by immunofluorescence (IF), remained practically unaltered after multiple freezing and thawing and after several weeks of storage at ––70 °C. In order to establish a reliable immunochemical base for quantified IF studies, the use of such particles is proposed. The role of particles in the determination of optimal working titers, specificity tests, or as a reference standard is discussed.
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