Abstract
The effect of the liquid junction potential produced by various salt bridges on blood pH determination was estimated using a new salt bridge consisting of the equilibrium diffusate of human plasma. Such a salt bridge should produce a state of ionic equitransference at the junction between itself and plasma and, except for a Donnan potential, no liquid junction potential should arise at this boundary. A mean pH of 7.41±0.01 was obtained with the pH cell incorporating an equitransferent liquid-liquid junction. This value was not significantly different from the mean pH of 7.42±0.01 obtained with the cell incorporating a KCl 3M salt bridge. However, a pH cell with NaCl 0.9% salt bridge yielded a mean pH of 7.30±0.01, a value significantly different from the other two cells. These results probably suggest that there is a significant liquid junction potential at the isotonic NaCl-blood boundary, and that concentrated KCl bridge electrolyte probably minimizes the liquid junction potential against blood.
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