Abstract
BackgroundLipoxygenases (LOXs) are important dioxygenases in cellular organisms. LOXs contribute to plant developmental processes and environmental responses. However, a systematic and comprehensive analysis has not been focused on the LOX gene family in poplar. Therefore, in the present study, we performed a comprehensive analysis of the LOX gene family in poplar.ResultsUsing bioinformatics methods, we identified a total of 20 LOX genes. These LOX genes were clustered into two subfamilies. The gene structure and motif composition of each subfamily were relatively conserved. These genes are distributed unevenly across nine chromosomes. The PtLOX gene family appears to have expanded due to high tandem and low segmental duplication events. Microarray analysis showed that a number of PtLOX genes have different expression pattern across disparate tissues and under various stress treatments. Quantitative real-time PCR (qRT-PCR) analysis was further performed to confirm the responses to MeJA treatment of the 20 poplar LOX genes. The results show that the PtLOX genes are regulated by MeJA (Methyl jasmonate) treatment.ConclusionsThis study provides a systematic analysis of LOX genes in poplar. The gene family analysis reported here will be useful for conducting future functional genomics studies to uncover the roles of LOX genes in poplar growth and development.
Highlights
As an important tree species of shelterbelt and timber forest in our country, poplar trees have enormous economic and ecological benefits, as well as unique biological properties of basicPLOS ONE | DOI:10.1371/journal.pone.0125526 April 30, 2015Analysis of LOX Genes in Poplar
Quantitative real-time PCR analysis was further performed to confirm the responses to Methyl Jasmonate (MeJA) treatment of the 20 poplar LOX genes
The results show that the PtLOX genes are regulated by MeJA (Methyl jasmonate) treatment
Summary
Lipoxygenases (LOXs) are important dioxygenases in cellular organisms. LOXs contribute to plant developmental processes and environmental responses. A systematic and comprehensive analysis has not been focused on the LOX gene family in poplar. In the present study, we performed a comprehensive analysis of the LOX gene family in poplar
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
