The Limited Domain of the Law

Development of sensor proteins for intracellular imaging of transition metals

PT Insan Persada as a company engaged in the field of waterproofing contractor with a broad scope of projects and many are located outside the city requires optimal IT involvement so the need for a mechanism appropriate IT governance standards. COBIT 4.1 framework provide solutions to implement IT governance model. This study focuses on the domain of the domain that washy and organise PO4 (determining the IT processes, organization and relationship), PO5 domains (managing IT investments), PO7 domain (IT human resource management), domain PO8 (managing quality), and the domain PO9 (assess and manage IT risks). This research using the method management awareness and maturity level in each domain of each PO4, PO5, PO7, PO8 and PO9. Based on calculations using the performance level of management awareness on PO4 domain has an average value of 1:22 (approximately), PO5 domain with an average value of 1.90 (moderate), PO7 domain with an average value of 1:46 (approximately), with a mean value PO8 domain average 1.67 (medium) and PO9 with an average value of 1:38 (approximately). While the calculation of the level of maturity assessment using the domain PO4 maturity level is 1 (initial/ad hoc), PO5 domain is 2 (repeatable but intuitive), PO7 domain is 2 (repeatable but intuitive), PO8 domain is 2 (repeatable but intuitive) and domain PO9 is 1 (initial/ad hoc). From the results of these studies indicate each is still not optimal for each domain and each domain has a gap that must be addressed as a form of recommendation on the proposed research. Keywords: Plan and organise, Management Awareness, Maturity Level, Waterproofing

The so-called Bergman representation formula (reproducing formula) and various estimates for Bergman projection with positive reproducing kernel and sharp Forelli-Rudin type estimates of Bergman kernel are playing a crucial role in certain new extremal problems related to so-called distance function in analytic function spaces in various domains in Cn . In this paper based on some recent embedding theorems for analytic spaces in bounded domains with C2 boundary and admissible domains new results for Bergman-type analytic function spaces related with this extremal problem will be provided. Some(not sharp) assertions for BMOA and Nevanlinna spaces, for analytic Besov spaces in any D bounded domain with C2 boundary or admissible domains in Cn will be also provided. We remark for readers in addition the problems related to regularity of Bergman projection which we use always in proof in various types of domains with various types of boundaries (or properties of boundaries) are currently and in the past already are under intensive attention. Many estimates for reproducing operators and there kernels and Lp boundedness of Bergman projections have been also the object of considerable interest for more than 40 years. These tools serve as the core of all our proofs. When the boundary of the domain D is sufficiently smooth decisive results were obtained in various settings. Our intention in this paper is the same as a previous our papers on this topic, namely we collect some facts from earlier investigation concerning Bergman projection and use them for our purposes in estimates of distY (f, X ) function (distance function). Based on our previous work and recent results on embeddings in classical analytic spaces in domains of various type in Cn we provide several new general assertions for distance function in products of strictly pseudoconvex domains with smooth boundary, general bounded domains with C2 boundary and in admissible domains in various spaces of analytic functions of several complex variables.These are first results of this type for bounded domains with C2 boundary and admissible domains. In addition to our results we add some new sharp results for special kind of domains so called products of strictly pseudoconex domains with smooth boundary in Cn extendind our sharp results in strictly pseudoconex domains.

Netrin UNC-6 is a protein secreted from ventral cells that guides cell and growth cone migrations in Caenorhabditis elegans. Previously it was shown that UNC-6 domain V-2 regulates dorsal guidance activity and domain C regulates an activity that prevents the branching of axons when they respond to the N-terminal domains. Because these results indicate that the biological activities of UNC-6 are mediated through specific domains, we systematically examined each UNC-6 domain for guidance activities. Transgenic animals expressing UNC-6 derivatives with domain deletions and mutants with selective unc-6 loss-of-function mutations were analyzed. The results indicate that the VI, V-2, and V-3 domains are primarily required for dorsal migrations and the VI and V-3 domains are required for ventral migrations. These domains are likely important for responses mediated by the UNC-5 and UNC-40 receptors, respectively. Deletion of V-3 and a V-3 point mutation selectively affect either cell or growth cone migrations, indicating that each migration requires unique interactions with UNC-6. Deletion of domain VI or of a conserved eight amino acid motif within VI causes loss of all UNC-6 guidance activities, and mutations within domain VI selectively affect different guidance activities, suggesting that domain VI regulates each response to UNC-6. We propose that individual UNC-6 domains mediate different signals, which act in parallel to regulate the morphological changes necessary for guidance.

Villin is a modular protein that regulates F-actin bundles in the microvilli of absorptive epithelial cells in the intestine. At low (10-100 nM) calcium levels, Villin is an F-actin bundling agent supporting the specialized brush border membrane of the absorptive epithelium. At intermediate micromolar calcium levels, Villin nucleates and caps the barbed ends of F-actin and in high (> 100 μM) calcium Villin is an F-actin severing agent (Bretsher & Weber, 1980; Glenney et al., 1980, 1981; Mooseker et al. 1980). The amino acid sequence of Villin has seven modular domains. The first six Villin domains (D1-D6) form a “core” of ~50% sequence identity with Gelsolin; and contain a Ca 2+ -dependent actin-binding site associated with the D1-D3 fragment. The last domain, Villin’s unique C-terminal headpiece (HP), contains the other F-actin binding site, which is Ca 2+ -independent (Bretsher & Weber, 1980; Glenney et al., 1980, 1981; Mooseker et al. 1980). Recent investigation by Nuclear Magnetic Resonance (NMR) Spectroscopy and Negative-Stain Electron Microscopy (EM) of the backbone dynamics and actin-binding of Villin’s D6-HP, 208-residue, C-terminal modular fragment, revealed that: a) folded domains D6 and HP are interacting only via a largely unfolded 40-residue linker, and b) at millimolar calcium levels, the monomeric D6-HP fragment bundles F-actin and has two actin binding sites; one, which is previously known on HP, and the other is novel, cryptic and Ca 2+ -dependent, associated with domain D6 or the linker (Smirnov et al., 2007). We have investigated how the domain structure, domain-domain and linkerdomain interactions in D6-HP fragment of Villin define its actin regulation properties. v Toward this goal, we are: a) making the D6 and D6-HP NMR samples; b) determining the NMR resonance assignment of isolated D6; and c) elucidating the solution structure of D6 domain in isolation and within the D6-HP fragment. Our NMR data indicate that the D6 protein fragment in isolation likely adopts a Gelsolin-like fold and that HP and D6 structures in isolation resemble those in the context of the larger modular fragment D6-HP. The potential effect of the linker on the D6 and HP domains structure is exemplified by the noticeable chemical shift differences for residue 84 of D6 and residue 166 of HP ( 15 N-HSQC spectrum of D6-HP vs. D6 and HP in isolation). These two positions are ~23 residues away from either end of the linker and located on the surface of these domains. In the absence of calcium, Gelsolin adopts a compact, inactive conformation stabilized by the 12-residue C-terminal helix. This helix was suggested to keep together Gelsolin domains D2 and D6 as a “latch” closed in low calcium and released at higher calcium levels (Robinson et al., 1999). Our ensuing structural study of D6-HP will clarify whether the linker sequence in D6-HP corresponding to this C-terminal helix of Gelsolin forms a helix as well and thus may or not undergo a gelsolin-like, calciuminduced rearrangement. The solution structure of D6 will be determined by NMR and analyzed in combination with the complete solution structure of HP and known structural properties of D6-HP. Together with the calcium and F-actin binding properties of D6 in isolation (currently under study), these data will clarify the role of the C-terminal domains of Villin in its activity as a physiologically principal actin regulator of microvilli.

Objective: To verify user satisfaction with the public oral health services in its different dimensions of care and relate them to the socioeconomic and demographic conditions. Material and Methods: Cross-sectional survey, developed with users interviewed of the public oral health services under the Primary Health Care of a Brazilian city (n=461). The associations of satisfaction with the socioeconomic and demographic conditions were analyzed using the chi-square test. Questions concerning satisfaction with the service were grouped into five domains - 'physical structure', 'relation and communication', 'information and support', 'health care' and 'organization of services' - analyzed using the Kruskal-Wallis Test . Results: Elderly individuals with low education and male, tended to be more satisfied with the services. The users reported being satisfied with the service, that were unhappy, referred to the time and the form to schedule a consultation, the time expected to be attended and the resolving capacity of oral health problems, as the causes. The overall satisfaction index was 0.8. The top rated domains were 'physical structure' (0.9) and 'relation and communication' (0.85). The 'service organization' domain received the worst evaluation (0.71) and when compared to the others domain presenting a significant difference. The 'physical structure' domain was significantly highlighted from the domains of 'oral health care' (0.78) and 'information and support' (0.78). The 'relation and communication' domain, also significantly excelled over the areas of 'oral health care'. Conclusion: The users rated the aspects of health care in a very positive way. The areas of highest user satisfaction were 'physical structure' and 'relation and communication'. However, the need for directing production processes in health became evident, especially regarding the organization of services, the domain with the worst rating.

Identification of Key Structural Elements of ATP-Dependent Molecular Motors Yuan Zhang Molecular motors perform diverse functions in cells, ranging from muscle contraction, cell division, DNA/RNA replication, protein degradation, and vesicle transport. The majority of molecular motors use energy from the ATP hydrolysis cycle, converting chemical energy into mechanical work in cells. All ATP-dependent molecular motors have a similar ATP binding site, although the functions can be drastically different. Myosins comprise a large group of ATP-dependent molecule motors. The structure-function relationship governing different functions for different myosin families remains elusive. Hypothesizing that members of each family possess conserved residues for their consensus functions and residues distinctive from those of other families to differentiate their functions from functions of other myosin families, we developed an algorithm for comparative sequence analysis in a phylogenic hierarchy to identify family-specific residues for 38 myosin families/subfamilies that comprise human myosin members. We found a number of family-specific residues that have been reported, such as residues in β-cardiac myosin associated with hypertrophic cardiomyopathy and residues in myosin 7A associated with hereditary deafness. We also identified distinct features among myosin families that have never been reported, including a unique signature of the SH1 domain in each of the myosin families, residues differentiating αand β-cardiac myosins, and a unique converter domain of myosin VI. We further examined myosin VI to understand why it moves toward the (-)-end of actin filaments, opposite to the direction of all other myosins and to shed light on their links to prostate cancer and ovarian cancer, where myosin VI is over-expressed. We found that many of myosin VI specific residues locate in or adjacent to the converter domain, including a cluster of unique residues at the interface between the motor domain and the converter. Using molecular dynamics (MD) simulation, we found mutations of M701 on the SH1 helix and F763 on a helix of the converter caused the separation of the motor domain and the converter, indicating their important roles in linking the converter and the motor domain in the pre-power stroke state structure, potentially critical for positioning of lever arm. Using the location of the unique residues at the interface of the motor domain and the converter as the site of drug docking, we identified a set of candidate small molecules binding to this unique binding site selectively, potentially blocking the converter rotation of myosin VI. A benzoic acid (C15H17N3O3) was found to have the best score in docking, binding to both the converter and motor domain stably in a 200 ns MD simulation run. This molecule can be a good lead to be optimized to inhibit myosin VI functions in cancer patients. We have also applied our algorithm to other ATP-dependent molecular motors, including hepatitis C virus NS3 helicase and DEAD box helicase Mss116. We found an important residue, T324, in NS3 helicase connecting domains 1 and 2 acting as a flexible hinge for opening of the ATP-binding cleft and an atomic interaction cascade from T324 to residues in domains 1 and 2 controls the flexibility of the ATP-binding cleft in NS3 helicase. We also found a conserved flexible linker for Mss116, and the tight interactions between the Mss116-specific flexible linker and the two RecA-like domains are mechanically required to crimp RNA for the unique RNA processes of yeast Mss116.

Optical technologies are ubiquitously used in hi-tech devices. As a common feature of such devices one finds structures with dimensions in the order of the wavelength of the used light. To design and produce such devices, the wave nature of light must be taken into account. Accordingly, robust simulation tools are required which are based on rigorously solving Maxwell's equations, the governing equations of light propagation within macroscopic media. This thesis contributes to the modeling and the numerical computation of light scattering problems: Light scattering problems are typically posed on the entire space. The Perfectly-Matched -Layer method (PML) is widely used to restrict the simulation problem onto a bounded computational domain. We propose an adaptive PML method which exhibits a good convergence even for critical problems where standard PML implementations fail. Besides the computation of the near field, that is the electromagnetic field within the computational domain, it is of major interest to evaluate the electromagnetic field in the exterior domain and to compute the far field. So far, this was numerically only possible for simple geometries such as homogeneous exterior domains or layered media. To deal with more complicated devices, for example with waveguide inhomogeneities, we develop an evaluation formula based on the PML solution which allows for an exterior domain field evaluation in a half space above the device. Finally, we generalize the PML method to problems with multiply structured exterior domains. The term “multiply structured exterior domain” is defined in this thesis and means that the exterior domain exhibits several half-infinite structures. Mathematically, this gives rise to various complications. For example, no analytical solutions to Maxwell's equations for standard light sources are available in the exterior domain, which are needed to describe the incoming field in a light scattering problem. To tackle this we propose a new light scattering problem formulation which fits well into the PML method framework and which may be regarded as an extension of classical contributions by Sommerfeld, Wiener and Hopf. An exterior domain evaluation formula for multiply structured exterior domains with an extended illumination is derived as well.

This thesis reports the cloning, overexpression and characterization of two interesting proteins from Helicobacter pylori: Heat Shock protein A (HspA), and CoaX (Type III Pantothenate kinase). Helicobacter pylori produces an unusual GroES homologue protein referred as to HspA (Heat-shock protein A). Besides its classical co-chaperone activity, HspA plays additional roles being involved in nickel binding. It also exhibits an extended subcellular localization, ranging from cytoplasm to bacterial cell surface. In fact, unlike its homologue proteins, HspA is highly immunogenic being also present in the extra cellular media. For this reason it is considered a target for new therapeutic strategies. HspA consists of two domains: an N-terminal domain (domain A, residues 1-90), that is homologous with other GroES bacterial proteins and a C-terminal domain (domain B, residues 91-118), which other GroES-like proteins lack. Domain B is unique to HspA and contains 8 histidines and 4 cysteines which have been suggested to be involved in nickel binding.This study points on a unique characteristic of HspA among all GroES proteins: a high content of cysteine residues. Cysteine is the less represented residue in all known GroES proteins examined. In this context we have produced and characterized a recombinant HspA and its mutants Cys94Ala and Cys94Ala/Cys111Ala and we have identified the disulphide bridge pattern of the protein. In particular the study has been addressed on the Cys oxidised/reduced state; the disulphide bridge pattern has been assigned by integrating classical biochemical methodologies with mass spectrometry. We found that the cysteines (two from domain A and four from domain B) are engaged in three disulphide bonds between residues Cys51/Cys53, Cys94/Cys111 and Cys95/Cys112. Our results suggest that two of the disulphide bridges, located in the B domain, force the C-terminal domain to adopt a unique closed loop structure that would be optimal for binding to 2 Ni ions as suggested by the different redox environments that the protein experiences inside and outside the bacterial cell.H.pylori also produces a new Pantothenate Kinase isoform (HpCoaX) encoded by the CoaX gene referred as to HP0862, involved in a critical pathway for pathogen survival: that of coenzyme A (CoA) biosynthesis. In particular, pantothenate kinase catalyzes the first step of the universal five-step CoA biosynthetic pathway. HpCoaX enzyme, belonging to the type III class, differs in sequence, structure and enzymatic properties from the previously characterized type I, found essentially in bacteria, and type II forms found in eukarya as well as in some bacteria.In this context we have cloned the HpCoaX gene in pRoEX-HTc vector and expressed the recombinant protein in E.coli BL21( DE3) cells. We have optimized the experimental conditions for purification and stability of the expressed protein. The purified HpCoax was analyzed by gel filtration chromatography and dynamic light scattering to investigate its state of oligomerization. Results show that HpCoaX exists as a homodimer, as predicted by its homology with the other PanK III bacterial proteins. H. pylori infections remain a significant global public health problem. Vaccine and antagonist compound development against this infection appears to be a preferable strategy. Actually there is a substantial requirement for new drug targets as alternative strategies for the treatment of H. pylori infections, since there is often resistance against traditional antibiotic therapy.For the all above reasons, both HpCoaX and HspA can be considered to be a good targets for new therapeutic strategies. Our study belongs to a wide research line with the aim of identifying, characterizing and clarifying the role of specific targets, in particular of multifunctional uncharacterized proteins involved in critical pathway for the pathogen survival.

Sharing information between logical variables is crucial for a lot of analyses of logic programs, e.g., freeness analysis, detection of And-parallelism, and occur-check. Therefore, the development of accurate sharing domains has attracted a lot of research. The sharing domain bf JL of Jacobs/Langen, which represents substitutions by powersets of variables, is considered one of the most precise sharing domains. However, it is too inefficient in practice; lots of programs cannot be analyzed in reasonable time. Improvements of bf JL, by adding auxiliary information like linearity, suffer from the same inefficiency, too. To improve upon this situation, we systematically derived a new sharing domain mathorddownarrowbf JL from bf JL which represents variables by downward closed powersets of variables. We combined mathorddownarrowbf JL with the groundness domain bf POS. Both bf JL and the new domain mathorddownarrowbf JL+bf POS have been implemented with the help of the Prolog analyzer generator GENA. In order to study the impact of linearity, we also implemented the abstract domains bf JL+bf LIN and mathorddownarrowbf JL+bf POS+bf LIN. The new domains are much more efficient as their counterparts bf JL and bf JL+bf LIN, respectively. Even more important, they can analyze even largest real-world programs in reasonable time. Surprisingly, the new sharing domains seem to have the same precision than bf JL and bf JL+bf LIN in practice.

This study aimed to investigate the reality of compatibility in its four domains, i.e. social, academic, disciplinary, and emotional among students of Al Ain University in the UAE. It also aimed to know the differences in the reality of compatibility according to the variables under study and the interaction between them (college, gender, change of specialization, and residence in the place of the university), to achieve the objectives of the study, the two researchers used the University Compatibility Scale. The sample consisted of (422) male and female students, including (173) male and (249) female students in the second semester 2019/2020. The results showed that the reality of compatibility in all its four dimensions is positive among the sample members, as in the first rank came the social domain at a ratio (74.8%), followed by the emotional (56.47%), then disciplinary (54.80%), and finally the academic (51.53%). The study results also revealed no statistically significant differences in the reality of compatibility in each domain (social, academic, and disciplinary) in the college variable. It was clear that there are differences in the emotional domain in favor of the human faculties and the absence of differences in the two domains, i.e. social and emotional in the gender variable. The study also resulted in the absence of differences in the disciplinary domain in the variable of change of specialization, while the existence of statistically significant differences in the domains (social, academic and emotional) according to the variable of change of specialization, in favor of students who did not change their specialization in the social and academic domains, and the overall degree of compatibility. The results also indicated that there are no differences in the residency in the location of the university variable in the two domains, i.e. disciplinary and emotional, while the differences in the two domains, i.e. social and academic are in favor of students who reside in the location of the university, in light of the results of the study, some recommendations were mentioned.

In this paper we examine how discourses are mobilised and deployed by actors in a domain during a critical incident. In particular we examine the changes and the continuities in the way that the major parties in the Melbourne port dispute of 1997-98 related to each other. We focus on the discourses used to structure the ways that these organisations related. We adopt perspectives from critical domain theory and discourse theory, and derive our main concept and unit of analysis, what we call the discursive domain. The main process that occurs in a discursive domain is actors' 'mapping' discourses onto concepts, and concepts onto objects, in order to make certain courses of action rational and sensible. We propose that different mapping processes occur in differently organised domains. We employ case study method. Data was gathered from an analysis of media reports about the port of Melbourne during 1997-98. These texts are used to examine the kinds (number and diversity) of discourses used by each of two main networks of organisations in the domain during that period. The main finding is an unexpected association between the 'organised' domain and the 'complex' mapping performed by actors. Our contribution is to build on previous understandings of the role of discourses in domains by examining the complexity of 'mapping' by domain actors during critical incidents such as a major industrial dispute. We conclude by drawing implications of the discursive domain approach for domain theory and discourse theory.

Protein domains are highly conserved functional units of proteins. Because they carry functionally significant information, the majority of the coding disease variants are located on domains. Additionally, domains are specific units of the proteins that can be targeted for drug delivery purposes. Here, using information about variants sites associated with diseases, a disease network was built, based on their sharing the same domain and domain variation site. The result was 49,990 disease pairs linked by domain variant site and 533,687 disease pairs that share the same mutated domain. These pairs were compared to disease pairs made using previous methods such as gene identity and gene variant site identity, which revealed that over 8,000 of these pairs were not only missing from the gene pairings but also not found commonly together in literature. The disease network was analyzed from their disease subject categories, which when compared to the gene-based disease network revealed that the domain method results in higher number of connections across disease categories versus within a disease category. Further, a study into the drug repurposing possibilities of the disease network created using domain revealed that 16,902 of the disease pairs had a drug reported for one disease but not the other, highlighting the drug repurposing potential of this new methodology.

High coupling materials for thin film bulk acoustic wave resonators

With the development of remotely sensed data acquisition techniques, the integration of complementary data has found a key role in many applications. The prerequisite of the data integration is the data registration. There are many approaches for image registration that they can be categorized based on the nature of data sources as the multi domain and single domain methods. The multi domain methods employ the data sources with different properties of gray values such as irradiance, the return strength of the laser pulse, and the height values. The different aspects of multi domain registration methods include the matching algorithm as feature-based or intensity-based, the level of automation, availability of initial registration parameters, the implementation cost, and so on. In this study, a number of multi domain registration algorithms are selected and compared based on the mentioned aspects and then, an automatic multi domain registration method is proposed to register an intensity image of LiDAR and a satellite image without using initial registration parameters. For this, the combination of the Scale Invariant Feature Transform (SIFT) detector and the mutual information theory is employed to use the strengths of both feature-based and intensity-based matching algorithms to decrease the difficulty of multi domain image registration. The final registration results prove that the combination of the feature-based and the intensity-based matching algorithms could be an efficient solution of multi domain image registration problem, especially for registration between the LiDAR intensity and the satellite images with the RMSE of one pixel.