Abstract
The application of an atomic force microscope (AFM) based microcantilever system for the determination of mass of gold nanoparticles (AuNPs) has been demonstrated. In this system, standard AFM microcantilevers for measurements in vacuum have been employed. The limit of mass determination with our AFM-based system has been determined to be of the order of 10-10 g. The prospects of employing AFM cantilever-based sensors for highly sensitive protein detection in proteomic studies and in diagnostics have been discussed.
Highlights
In our present study, the sensitivity of a microcantilever system employing standard atomic force microscope (AFM) microcantilevers for measurements in vacuum, has been estimated
We have demonstrated that such a system allows one to register ~100 gold nanoparticles (AuNPs), what corresponds to 17 μL of 10-17 М solution of protein labeled with these nanoparticles
To measure the resonance frequency shift caused by adsorption of nanoparticles onto the cantilever, the following technique was employed
Summary
The sensitivity of a microcantilever system employing standard AFM microcantilevers for measurements in vacuum, has been estimated. We have demonstrated that such a system allows one to register ~100 gold nanoparticles (AuNPs), what corresponds to 17 μL of 10-17 М solution of protein labeled with these nanoparticles. To measure the resonance frequency shift caused by adsorption of nanoparticles onto the cantilever, the following technique was employed.
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