Abstract
Nyctinastic leaf-movement of the plant is controlled by a biological clock. The most difficult problem encountered in the chemical studies of nyctinasty, is the isolation of chemical signals controlling this movement. Now, we have established a good procedure for isolation of the chemicals causing leaf-movement. The separation of two inversely bioactive substances, causing leaf-closing and opening movement, by Amberlite XAD-7 chromatography was very effective. Final separation with HPLC using a combination of plural ODS columns enabled us to isolate the highly polar ieaf-closing and -opening substances of Albizzia julibrissin Durazz.
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