The isolation of acid phosphatase isoenzymes of Oncopeltus fasciatus, (Dallas) (Hemiptera: Lygaeidae)

Abstract

1. 1. Acid phosphatase isoenzymes of selected ages of fourth, fifth, and adult stage milkweed bugs were isolated by CM-cellulose and DEAE-cellulose chromatography and gel electrophoresis. 2. 2. Acid phosphatase-I (AP-I) and acid phosphatase-II (AP-II) were isolated by CM-cellulose chromatography and were found in all examined bugs. 3. 3. Day 14 adult females gave a third isoenzyme, AP-III, from the CM-cellulose column. When acid phosphatase-III (AP-III) was applied to the DEAE-cellulose column, three additional isoenzymes, acid phosphatase-III-1 (AP-III-1), acid phosphatase-III-2 (AP-III-2), and acid phosphatase-III-3 (AP-III-3), were isolated. 4. 4. Gel electrophoresis separated acid phosphatase-I (AP-I) into two isoenzymes, AP-I-1 and AP-I-2, in samples from all groups except the Day 14 adult male which had only AP-I-1 on gels run at pH 8.3. 5. 5. Acid phosphatase-II (AP-II) was a single diffuse band in all groups on gels run at pH 4.5. 6. 6. Acid phosphatase-III-1 (AP-III-1), acid phosphatase-III-2 (AP-III-2), and acid phosphatase-III-3 (AP-III-3) from Day 14 adult females gave each a single diffuse band on gels run at pH 4.5. 7. 7. Juvenile hormone application to the fifth instar bugs had no effect on acid phosphatase activity, total protein, or the electrophoretic separation of acid phosphatase isoenzymes of the fifth instar bugs when compared to the acetone controls.