Abstract
1. 1. Asters produced in artificially activated eggs can be isolated by the same methods which have been used to isolate the mitotic apparatus from spermfertilized eggs. The methods described were the alcohol-digitonin and the sucrose-versene-dithiodiglycol methods. 2. 2. These isolated asters have similar solubility and stability properties as those of the mitotic apparatus. Isolated asters can be dissolved in Salyrgan (mersalyl acid) at pH 9, and in 0.53 M KC1 at pH ca 8. 3. 3. Ultracentrifuge analysis of SVD-isolated asters shows two peaks, with sedimentation velocities of 14 and 21. 4. 4. Electrophoresis of SVD-isolated asters shows two peaks; the major peak has a mobility of 1.04 × 10 −4 cm 2/volt sec, and the minor peak a mobility of 7.2 × 10 −5 cm 2/volt sec. 5. 5. Absorption spectra (UV) of solubilized SVD and digitonin-isolated asters show maxima at 260 mμ. 6. 6. ATPase analysis of dissolved asters gives an activity lower than that shown for the mitotic apparatus. 7. 7. Immunochemical identity has been found between isolated mitotic apparatus from sperm-fertilized eggs, and asters from artificially activated eggs.
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