Abstract
Tetrastigma hemsleyanum is traditionally used as a folk medicine and functional food in China. Its extracts have been confirmed to have many bioactivities. However, the effect of extracting temperature on its bioactivity has not been reported. In this research, the total flavonoids content (TFC), total polyphenol content (TPC), antiproliferative, antioxidant, and anti‐inflammatory activities of ethanol extracts and water extracts (extracted at 55, 70, 85, and 100°C) were observed. The results indicated that ethanol extracts showed better antioxidant activity with DPPH EC50 of 504.1 ± 3.8 μg/ml and ABTS EC50 of 851.4 ± 3.9 μg/ml. A better antiproliferative activity on HepG2, PC12, Caco‐2, and Hela cells was observed on ethanol extracts. The results of anti‐inflammatory activities also indicated that all of the extracts can reduce the NO production of LPS‐stimulated macrophage with dose‐independent manner. In summary, the results showed that the antiproliferative, antioxidant, and anti‐inflammatory activities of water extracts decreased with the increasing temperature to some extent.
Highlights
As many natural products are confirmed to have many bioactivities, such as antioxidant and anti‐aging activities, the extracts of natural products captured many researchers' attention (Chen, Zhang, Chen, Han, & Gao, 2017)
All of the three assays of DPPH, azino‐bis(3‐ethylbenzothiazoline‐6‐sulfonate) cation (ABTS), and ferric reducing antioxidant power (FRAP) indicated that the ethanol extracts had the best antioxidant activity and antioxidant activity of water extracts in vitro decreased with increasing temperature (p < .05)
The results suggested total flavonoids content (TFC) and total polyphenol content (TPC) of water extracts decreased with the increasing extracting temperature and the lowest TFC and TPC were obtained at THW100 with the value of 216.0 ± 4.0 mg rutin/g DW and 90.6 ± 2.9 mg gallic acid/g DW
Summary
As many natural products are confirmed to have many bioactivities, such as antioxidant and anti‐aging activities, the extracts of natural products captured many researchers' attention (Chen, Zhang, Chen, Han, & Gao, 2017). The composition and bioactivities of extracts of natural products were usually affected by the extracting method, solvent, and temperature (Cvetanović et al, 2019; Gayathri et al, 2019; Pimentel‐moral et al, 2018; Sun et al, 2016; Wang, Liu, & Hu, 2014; Yu, Ji, Yang, & Liu, 2019; Zhang et al, 2016). Tetrastigma hemsleyanum Diels et Gilg belongs to the genus Tetrastigma Planch, family Vitaceae. Its root and whole grass have many bioactivities which can be used as herbal medicine (Sun et al, 2018, 2013, 2017) It is especially famous for its root which is widely used for removing pathogenic heat from blood, activating blood, and alleviating pain. Modern pharmacological studies show that T. hemsleyanum has a wide range of pharmacological functions, such as antiproliferative, antivirus, anti‐inflammatory, antidiabetic, liver protective, and immunoregulation activities
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
