Abstract
A method for the preparation of sub-mitochondrial particles from Jerusalem artichoke tubers by sonication is described. The particles carried out a rapid oxidation of NADH, succinate, and ascorbate saturated with N',N',N, A-tetramethylphenylenediamine (TMPD). The difference spectrum (dithionite reduced minus oxidized) of sub-mitochondrial particles was similar to that of whole mitochondria. In the autumn phosphorylation accompanied electron transport only when supernatant from the preparation procedure was added to the sub mitochondrial particles in the presence of magnesium. In the winter and spring there was a decrease in the effect of supernatant on phosphorylation, and the sub-mitochondrial particles alone synthesized ATP during substrate oxidation. It is suggested that the reconstitution of phosphorylation in Jerusalem artichoke sub-mitochondrial particles is essentially similar to that observed in beef heart electron transport particles, but that phosphorylation in the former is subject to seasonal variations.
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