Abstract

Yogurt is a nutritious dairy product, has established probiotics effects and may even be therapeutic for certain health conditions. Proteolytic activity of starter cultures during milk fermentation can be generating peptides with angiotensin-I converting enzyme (ACE-I; EC. 3.4.15.1) inhibitory activity. Effects of presence ofAllium sativumorCinnamomum verumon these peptides in yogurt were investigated. Two types of milk (cow and camel) were incubated (41°C) with starter culture in the presence ofA. sativumorC. verumwater extract until pH of yogurt was reduced to 4.5. The resulting herbal-yogurts were evaluated with respect to changes ofin vitroACE-I inhibitory activity and proteolytic activity during 21 days of storage. In the present study, the extent of proteolysis was higher (p<0.05) in presence ofA. sativumthan presence ofC. verumduring storage with cow milk yogurt being more susceptible than camel-milk yogurt. The presence ofA. sativumorC. verumincreased ACE-I inhibitory activity more in cow-than camel-milk yogurt during storage. All herbal-yogurts showed the highest inhibitory activity of ACE-I (40%- 70%) on day 7 of storage. Prolonged storage of both types of yogurt to more two weeks decreased ACE-I inhibition activity (37% - 50%; p<0.05). In conclusion,A. sativumandC. verumcan enhance yogurt functional properties with potential therapeutic values for hypertension.

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