Abstract
Normal human IMR 90 fibroblasts at population doubling levels (PDL) 28 approximately 33 had an increased incorporation of 3H-thymidine into DNA when the cells were pretreated with physiologic concentrations of insulin (5 approximately 10 ng/ml) prior to re-initiation of the cell cycle. Insulin stimulation was not observed with fibroblasts at PDL 50 nor with SV40 virus transformed cells. In all cells treated with supra-physiologic concentrations of insulin (1000 approximately 6000 ng/ml) prior to the re-initiation of cell cycle, the incorporation of thymidine into DNA was greatly diminished. Autoradiographic studies of the effect of insulin suggest that a larger fraction of cells was found to enter S phase than untreated cultures. This effect was evident in younger cells rather than senescent cells.
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