The Influence Of Annona Muricata Leaves Extract In Damaging Kidney Cell And Inducing Caspase-9 Activity

Abstract

Annona muricata leaf extract is used in Indonesia traditional medicine for the treatment of many disease . Work done has demonstrated Annona muricata to be effective in treating carbon tetrachloride-and acetaminophen-induced hepatic damage, In this present study, the efficacy of ethanolic extracts of Annona muricata leaves for its cytotoxicity potential and induction of apoptosis in tubular cells, was investigated. Phytochemical screening verified presence of alkaloids, tannins, flavonoids, saponins, anthraquinones and cardiac glycosides. The present study was undertaken to investigate the leaf extract effects of Annona muricata Linn. on the level of serum creatinine and damage of tubular cell structure that effect of kidney cell functions and caspase-9 expression in glomerulus and tubular cells. The animals (n=20) Were grouped a (control), I, II, AND III (experimental). The experimental animals of group I were administered with 10 mg/kg body weight/ day annona muricata extract in saline for 40 days, group II were administered with 20 mg/kg body weight/ day annona muricata extract in saline for 40 days and III were administered with 40 mg/kg body weight/day annona muricata extract in saline for 40 days and then we see the effect on creatinine serum concentration by ELISA method and glomerulus,tubular cells were fixed and processed to examine the histological changes and caspase-9 expression in glomerulus and tubular cells.