Abstract
P077 The intermediate state of HTLV-1 infection, often found in dual carriers of Strongyloides stercoralis (S. stercoralis). To investi-gate the effects of S. stercoralis superinfection in the natural history of HTLV-1 infection, we characterized the peripheral blood of the individuals dually infected with HTLV-1 and S. stercoralis. Immunophenotyping of the peripheral blood mono-nuclear cells (PBMC) revealed an increase in CD4+25+ T cell population in dually infected carriers compared with that in normal controls and single carriers of S. stercoralis or HTLV-1. Semiquantitative PCR analysis of PBMC DNA revealed that the HTLV-1 provirus load in dually infected carriers is higher than that in single carriers of HTLV-1. Analysis of the clonality of HTLV-1-infected cells in the PBMC using inverse-long PCR technique demonstrated polyclonal expansion of the HTLV-1-infected cells in dual carriers of HTLV-1 and S. stercoralis, as demonstrated in single HTLV-1 carriers. Activation of the promoter of IL-2 gene by Strongyloides antigen was shown in reporter gene assay with IL-2 CAT. Taken collectively, these results suggest that S. stercoralis infection and induction of IL-2 production may enhance polyclonal expansion of HTLV-1-infected cells in dually infected carriers.
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