Abstract
Background Fluorescence lifetime of NADH had been proposed to use as an intrinsic biomarker for monitoring cellular metabolism. In our pervious studies, we have demonstrated that NADH lifetime of hMSCs increase gradually with time of osteogenic differentiation. In this study, we performed NADH lifetime measurement of hMSCs from a different donor and studied the association with several metabolic indices such as ATP level, oxygen consumption and lactate release. We also measured the quantity of Complex I, III, IV and V during hMSC differentiation.
Highlights
Fluorescence lifetime of NADH had been proposed to use as an intrinsic biomarker for monitoring cellular metabolism
Materials and methods NADH fluorescence lifetime images were performed as our previous studies [1]
Time-resolved detection was conducted by the single-photoncounting SPC-830 printed circuit board (Becker & Hickl GmbH, Berlin, Germany)
Summary
Fluorescence lifetime of NADH had been proposed to use as an intrinsic biomarker for monitoring cellular metabolism. Materials and methods NADH fluorescence lifetime images were performed as our previous studies [1]. Treated hMSC cells were imaged with a two-photon laser scanning microscope and with a 60 × 1.45 NA PlanApochromat oil objective lens (Olympus Corp., Japan).
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