The in vivo and in vitro influence of methyl jasmonate on oxidative processes in Arabidopsis thaliana leaves

Abstract

In Arabidopsis thaliana leaves a strong increase of H2O2 content was induced by application of methyl jasmonate (JAMe) through the root system, but the induction only slightly depended on JAMe concentration. The activity of superoxide dismutase and ascorbic acid peroxidase increased at lower JAMe concentrations and decreased at higher ones. Catalase activity decreased proportionally to JAMe concentration (in comparison with control plants). The sum of ascorbic acid and dehydroascorbate content at 10−6 M JAMe was similar to the control, but at higher concentrations it increased, especially due to a higher ascorbate accumulation. Methyl jasmonate applied directly to the extract of leaves (in vitro experiment) also induced a strong increase in H2O2 level, even at a low concentration (10−8 M). Since lower JAMe concentrations induced weak superoxide dismutase and did not change catalase and peroxidase activity, it is suggested that in this case a high level of hydrogen peroxide was not the result of the activity of the mentioned enzymes. JAMe-induction of H2O2 increase at the highest JAMe concentration resulted from SOD activity. Our in vivo and in vitro experiments suggest that jasmonate can influence oxidative stress not only through gene expression but also by its direct effect on enzyme activity.