Abstract
BackgroundDevelopment in guided tissue regeneration requires biomaterial testing. 3D cell constructs represent a new approach to bridge the gap between cell culture and animal models. Following the hypothesis that attachment behavior of cells could be observed in toroidal 3D cell constructs, the aim of this study was to evaluate 3D gingival fibroblast (GF) toroids as a simple and feasible in vitro assay to test attachment of oral fibroblasts to collagen membranes.Methods3D ring-like structures (toroids) were formed from human GF. Hematoxylin-eosin staining was performed with formed GF toroids. Produced GF toroids were seeded onto plastic surfaces or collagen membranes. The morphology was documented at 24 h, 48 h and 72 h after seeding with light and fluorescence microscopy. Toroid vitality was assessed at same time points with a resazurin-based toxicity assay.ResultsGF showed normal morphology in toroid hematoxylin-eosin staining. Over 72 h, GF toroids on plastic surfaces stayed unchanged, while GF toroids on collagen membranes showed dilatation. GF toroids on plastic surfaces and collagen membranes were metabolically active over the observed period.ConclusionsDepending on the surface material, 3D GF toroids show different attachment behavior. Thus, GF toroids are suitable as simple assay to study attachment behavior to various biomaterials.
Highlights
Development in guided tissue regeneration requires biomaterial testing. 3D cell constructs represent a new approach to bridge the gap between cell culture and animal models
Hypothesizing that toroid microtissues could be used as simple assays to observe behavior of tissue on biomaterials, the aim of this study was to test if toroids of human gingival fibroblasts (GF) can be used as simple and feasible in vitro assay to test attachment behavior to collagen membranes
Attachment behavior and vitality of gingival fibroblast toroids The attachment score shows that two out of five GF toroids attached for 72 h to both, plastic and collagen membranes (Table 1)
Summary
Development in guided tissue regeneration requires biomaterial testing. 3D cell constructs represent a new approach to bridge the gap between cell culture and animal models. Development in guided tissue regeneration requires biomaterial testing. A wide range of biomaterials is in use for GTR approaches and further materials are tested and optimized. A material that is used as a guiding structure for GTR ideally is biocompatible, a barrier between soft and hard tissue, easy to handle and supports regeneration [2]. Collagen membranes are one of the most popular candidates for GTR and have been in clinical use for years [3]. They show potential for GBR applications as for example sinus augmentation [4], providing a basis for implant placement.
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