Abstract
Giant mudskippers (Periophthalmodon schlosseri) are amphibious fish and considered as endemic fish, so it can not be found in any other place and they live in a typical habitat in a tidal area with the mud of mangroves. Mudskipper These fish is relatively untapped as food source. This research aims to obtain the optimum conditions of enzyme concentration, pH, temperature, and hydrolysis incubation time on the degress of hydrolysis of alcalase enzyme in giant mudskipper (P. schlosseri). The production of fish protein hydrolysate was conducted by varying one parameter at a time to determine its effect without considering interactions with other parameters. The results obtained optimum hydrolysis at 1.5% alcalase concentration of protein weight, pH 7.5, temperature 50°C, and the duration of hydrolysis 2 hours. The degree of hydrolysis produced at the optimum condition was 67.44%.
Highlights
Giant mudskippers (Periophthalmodon schlosseri) are amphibious fish and considered as endemic fish, so it can not be found in any other place and they live in a typical habitat in a tidal area with the mud of mangroves
The use of 1.5% alcalase enzyme concentration increased the degree of hydrolysis from 55.62% to 61.07%
The results show the use of 1% alcalase enzyme concentration with 1.5% and 2% alcalase enzyme concentrations had significant difference on the average value of the degree of hydrolysis
Summary
Giant mudskippers (Periophthalmodon schlosseri) are amphibious fish and considered as endemic fish, so it can not be found in any other place and they live in a typical habitat in a tidal area with the mud of mangroves. These fish is relatively untapped as food source because of its morphology and its habitat in mudflats. The enzymatic hydrolysis has been widely used in various protein derived from different aquatic sources to produce functional fish protein hydrolysate.[5,6,7,8,9,10,11,12,13,14] One of the proteolytic enzymes used was alcalase enzyme. Alcalase is an endo-protease of the serine type with a broad
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