The halophyte gene ScVTC2 confers resistance to oxidative stress via AsA-mediated photosynthetic enhancement

Abstract

Various abiotic stresses commonly cause excessive production of reactive oxygen species (ROS) and result in oxidative stress, which challenges the physiological homeostasis of plants. Maintaining a delicate balance between ROS generation and removal is critical for plants to cope with stressful environments. Suaeda corniculata is a typical euhalophyte with strong tolerance to salt stress, but its mechanism of ROS detoxification to prevent oxidative stress is unknown. Here, a combined analysis of RNA-Seq and photosynthetic assays was performed on S. corniculata under oxidative stress to uncover the underlying mechanism that modulates oxidative tolerance. Our results showed that all genes involved in the pathway of ROS scavenging, especially the AsA-GSH pathway, were highly enriched under oxidative stress. Notably, VTC2 (GGPase), which functions in the L-galactose pathway of AsA synthesis, was significantly upregulated. Arabidopsis transgenic plants with heterologous expression of ScVTC2 showed elevated AsA and increased tolerance to oxidative stress. Furthermore, ScVTC2 also established better photosynthetic capacity in these plants upon oxidative treatment. Thus, ScVTC2 not only functioned as an effective ROS scavenger but also as a protector of the photosynthetic apparatus in S. corniculata and allowed plants to respond to and tolerate oxidative stress.