Abstract
SUMMARY In experiments on the survival of dog spermatozoa during deep-freezing to — 79°C. and thawing, 8 per cent v/v glycerol in the diluent gave better revival rates than 4 per cent, but it was necessary to centrifuge and resuspend all samples of the spermatozoa in a diluent free of glycerol before strong motility was seen. The basic diluent, composed of Ringer solution and phosphate buffer, was improved by the addition of fructose and bovine plasma albumin or egg lecithin. Very few spermatozoa survived deep-freezing after a 50-fold dilution in Ringer-phosphate containing glycerol and fructose.
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