The first record of smallscale codlet, Bregmaceros nectabanus Whitley, 1941 (Teleostei: Bregmacerotidae) in Montenegrin waters and a review of previous Mediterranean records

Microplastic distribution, abundance, and composition in the sediments, water, and fishes of the Red and Mediterranean seas, Egypt

A species of the right-eyed flatfish family Samaridae, Samariscus inornatus, was collected from the Gulf of Elat. This species was previously known only from three syntypes from the Gulf of Aden. The species is redescribed and a key to the species of Samariscus is presented. One specimen of the pleuronectid Pleuronectes platessa was collected from the Gulf of Elat. This species is distributed from the White Sea, along the coasts of Europe, to the northern area of the Atlantic coast of Morocco and the western and central areas of the Mediterranean Sea. The Adriatic Sea is the eastern limit of this Mediterranean distribution. It appears to be highly probable that this species has been introduced into the Gulf of Elat via ballast water of ocean-going vessels. Since only one specimen was collected, it is not known if this species is established in the Red Sea.

Distribution, abundance, and composition of microplastics in market fishes from the Red and Mediterranean seas in Egypt

Massive occurrences of jellyfish can cause direct impacts on the economy, especially on tourism and commercial fisheries. Translocation of jellyfish species by humans has caused damaging blooms in new habitats. Aurelia aurita s.l. has been introduced in many locations around the world. To test the potential success of Au. aurita s.l. in various habitats, scyphistomae from different climatic locations (Mediterranean, Red and Baltic Seas) were cultured individually for 201 days at three temperatures (14, 21 and 28 °C) with the same salinity, food and light. We tested the null hypotheses that there were no differences in survival or asexual reproduction (budding and strobilation) amongst populations [native (Mediterranean) and exotic (Red and Baltic)]. Survival of the three scyphistoma populations did not differ significantly across temperatures; however, the Red Sea group had lower survival at all temperatures than did the other populations. Most individuals strobilated at 14 °C. Red Sea scyphistomae strobilated more quickly than Baltic and Mediterranean Sea scyphistomae and produced the fewest ephyrae, whereas Baltic Sea scyphistomae produced the most. Our results indicate that Au. aurita from the Baltic or Red Seas introduced into the Northwest Mediterranean Sea would potentially persist and successfully asexually reproduce there. A new invader could even have greater asexual production than the local Au. aurita s.l. Establishment of the invaders could increase genetic variation of subsequent generations and increase their adaptability to environmental changes. Our results suggest that introduction of exotic Au. aurita s.l. populations could increase jellyfish blooms in the Mediterranean Sea.

Vulnerability to bioinvasions: Current status, risk assessment and management of ballast water through a regional approach – the Adriatic Sea

Autonomous Reef Monitoring Structures (ARMS) have been applied worldwide to characterize the critical yet frequently overlooked biodiversity patterns of marine benthic organisms. In order to disentangle the relevance of environmental factors in benthic patterns, here, through standardized metabarcoding protocols, we analyse sessile and mobile (<2mm) organisms collected using ARMS deployed across six regions with different environmental conditions (3 sites×3 replicates per region): Baltic, Western Mediterranean, Adriatic, Black and Red Seas, and the Bay of Biscay. A total of 27,473 Amplicon Sequence Variants (ASVs) were observed ranging from 1,404 in the Black Sea to 9,958 in the Red Sea. No ASVs were shared among all regions. The highest number of shared ASVs was between the Western Mediterranean and the Adriatic Sea (116) and Bay of Biscay (115). Relatively high numbers of ASVs (103), mostly associated with the genus Amphibalanus, were also shared between the lower salinity seas (Baltic and Black Seas). We found that compositional differences in spatial patterns of rocky-shore benthos are determined slightly more by dispersal limitation than environmental filtering. Dispersal limitation was similar between sessile and mobile groups, while the sessile group had a larger environmental niche breadth than the mobile group. Further, our study can provide a foundation for future evaluations of biodiversity patterns in the cryptobiome, which can contribute up to 70% of the local biodiversity.

The paper analyses statutory foundations applicable to ships requiring to be exempted from the mandatory installation of the ballast water management system. In particular, it deals with ships sailing on regular international lines but within the semi-closed seas, such as the Adriatic Sea. The legal framework is set out in the International Convention for the Control and Management of Ships’ Ballast Water and Sediments (BWM, 2004), the IMO Guidelines for Risk Assessment A-4 (G7), and the Croatian Ordinance on Ballast Water Control and Management, 2012 [Pravilnik o upravljanju i nadzoru balastnih voda]. The paper presents the model procedure to be followed by main stakeholders, mainly national maritime administrations and shipping companies. The paper outlines the main advantages and disadvantages of the whole process and analyses several cases of good practice. Furthermore, the main stakeholders in the approval procedure and their responsibilities are described, available risk assessment models are analysed, with the particular emphasis on the species-specific risk assessment method as the most preferred method. Finally, the need for cooperation between the involved states and their maritime administrations is considered. It is concluded that bilateral and regional cooperations are an essential element in preserving the marine environment of any sea area. In that respect, the proposed model of the BWM system exemption approval in closed and semi-closed seas, such as the Adriatic Sea, is essential for shipping companies connecting ports in different countries on regular routes. Although demanding in respect of efforts and time, a procedure is viable and may be carried out in due time and with satisfactory outcomes.

The Mediterranean Sea is considered a hotspot for bioinvaders. Nonetheless, information on non-indigenous benthic foraminifera is still fragmented. This study documents for the first time the presence along the northwestern Adriatic coast of the non-indigenous benthic foraminifera species Virgulinella fragilis, Grindell and Collen (1976). Due to the low abundance recorded in the study area, the presence of this species may represent an early colonization phase. We discuss the temporal and spatial patterns of V. fragilis arrival in the Mediterranean and Adriatic Seas, and we hypothesize stowaway transport (via ship fouling or ballast water) as the main introduction pathway. Morphological test analyses suggest that V. fragilis prefers a low oxygen content, consistent with the ecological requirements reported for this taxon in the literature. The application of Maximum Entropy (MaxEnt) modeling indicates that the key factor influencing the presence of V. fragilis in the Mediterranean basin is the bacterial concentration expressed as NO3. Projections under future climate scenarios (RCP 4.5) point to a decline of habitat suitability conditions, making widespread invasion unlikely in the Mediterranean. We emphasize the importance of continuous biomonitoring for early detection of alien species, improving our understanding of invasion dynamics and enabling prompt conservation actions, especially in regions impacted by anthropogenic activities.

The re-examination of marine alien species or Non-indigenous species (NIS) reported in Italian Seas by December 2018, is here provided, particularly focusing on establishment success, year of first record, origin, potential invasiveness, and likely pathways. Furthermore, their distribution is assessed according to marine subregions outlined by the European Union (EU) Marine Strategy Framework Directive: Adriatic Sea (ADRIA), Ionian Sea and Central Mediterranean Sea (CMED), and Western Mediterranean Sea (WMED). In Italy, 265 NIS have been detected with the highest number of species being recorded in the CMED (154 species) and the WMED (151 species), followed by the ADRIA (143). Most of these species were recorded in more than one subregion. The NIS that have established stable populations in Italian Seas are 180 (68%), among which 26 have exhibited invasive traits.Among taxa involved, Macrophyta rank first with 65 taxa. Fifty-five of them are established in at least one subregion, mostly in the ADRIA and the CMED. Crustacea rank second with 48 taxa, followed by Polychaeta with 43 taxa, Mollusca with 29 taxa, and Pisces with 28 taxa, which were mainly reported from the CMED. In the period 2012-2017, 44 new alien species were recorded, resulting in approximately one new entry every two months. Approximately half of the NIS (~52%) recorded in Italy have most likely arrived through the transport-stowaway pathway related to shipping traffic (~28% as biofoulers, ~22% in ballast waters, and ~2% as hitchhikers). The second most common pathway is the unaided movement with currents (~19%), followed by the transport-contaminant on farmed shellfishes pathway (~18%). Unaided is the most common pathway for alien Fisshes, especially in CMED. Escapes from confinement account for ~3% and release in nature for ~2% of the NIS. The present NIS distribution hotspots for new introductions were defined on the first recipient area/location in Italy. In ADRIA the hotspot is Venice which accounts for the highest number of alien taxa introduced in Italy, with 50 newly recorded taxa. In the CMED, hotspots of introduction are the Taranto and Catania Gulfs, hosting 21 first records each. The Strait of Sicily represents a crossroad between the alien taxa from the Atlantic Ocean and the Indo-Pacific area. In the WMED, hotspots of bioinvasions include the Gulfs of Naples, Genoa and Livorno.This review can serve as an updated baseline for future coordination and harmonization of monitoring initiatives under international, EU and regional policies, for the compilation of new data from established monitoring programs, and for rapid assessment surveys.

Ballast water is recognized as a major vector for the transfer of Harmful Aquatic Organisms and Pathogens (HAOP) and a source of sea pollution that negatively affects the environment and human health. Therefore, the International Maritime Organization (IMO) adopted the International Convention for the Control and Management of Ship’s Ballast Water and Sediments (BWM Convention) in 2004. The BWM Convention introduced two standards, Ballast Water Exchange Standard (Regulation D-1) and Ballast Water Performance Standard (Regulation D-2). Ships are required to install Ballast Water Treatment (BWT) equipment in order to comply with Regulation D-2. However, the deadline for the installation of BWT is prolonged until September 2024, and many ships are still complying only with Regulation D-1. In addition, there are specific sea areas where Regulation D-1 cannot be complied with, and hence, HAOP could be easily transferred between ports. Consequently, it is essential to develop a system to protect the marine environment, human health and economy in coastal areas from the introduction of HAOP. This paper analyses ballast water discharged in the Port of Ploče (Croatia) according to ship type, age and flag they are flying. It was found that general cargo ships and bulk carriers discharged most of the ballast (87% of the total quantity) in the Port of Ploče. Moreover, discharged ballast water was analysed according to the origin, and it was found that 70% of discharged ballast originates from the Adriatic Sea. Based on the analysis of the research results and literature review, the ballast water risk assessment (BWRA) method was adopted, however, with certain modifications. The adopted method is modified by an additional risk factor (the deballasting ship’s age), different risk scoring of the deballasting ship type and adding Paris MoU Grey and Black lists flag ships as high-risk ships. As a result, the BWRA method presented in the paper could be used as an early warning system and to facilitate the implementation of adequate measures to prevent pollution by discharged ballast water.

The implementation of the ballast water management convention in the Adriatic Sea through States' cooperation: The contribution of environmental law and institutions

Ballast water management system: Assessment of chemical quality status of several ports in Adriatic Sea

We propose a basin-scale (∼300 × 100 km) study of the pre-salt to salt sedimentary fill from the Suez rift based on outcrop and subsurface data. This study is a new synthesis of existing and newly acquired data using an integrated approach with (1) basin-scale synthesis of the structural framework, (2) stratigraphic architecture characterization of the entire Suez rift using sequence stratigraphy concepts, (3) lithologic maps reconstruction and interpretation, (4) isopach/depocenter maps interpolation to quantify sedimentary volumes, and (5) quantification of the sediment supply, mean carbonate and evaporite accumulation rates, and their integration into the rift dynamic. The Gulf of Suez is ca. 300-km-long and up to 80-km-wide rift structure, resulting from the late Oligocene to early Miocene rifting of the African and Arabian plates. The stratigraphic architecture has recorded five main stages of rift evolution, from rift initiation to finally tectonic quiescence characterized by salt deposits. Rift initiation (ca. 1–4 Myr duration): the Suez rift was initiated at the end of the Oligocene along the NNW-SSE trend of the Red Sea with evidences of active volcanism. Continental to lacustrine deposits only occurred in isolated depocenters. Sediment supply was relatively low. Rift widening (ca. 3 Myr duration): the rift propagated from south to north (Aquitanian), with first marine incursions from the Mediterranean Sea. The rift was subdivided into numerous depocenters controlled by active faults. Sedimentation was characterized by small carbonate platforms and associated sabkha deposits to the south and shallow open marine condition to the north with mixed sedimentation organized into an overall transgressive trend. Rift climax (ca. 5 Myr duration): the rift was then flooded during Burdigalian times recording the connection between the Mediterranean Sea and the Red Sea. The faults were gradually connected and reliefs on the rift shoulders were high as evidenced by a strong increase of the uplift/subsidence rates and sediment supply. Three main depocenters were then individualized across the rift and correspond to the Darag, Central, and Southern basins. Sedimentation was characterized by very large Gilbert-type deltas along the eastern margin and associated submarine fans and turbidite systems along the basin axis. Isolated carbonate platforms and reefs mainly occurred in the Southern basin and along tilted block crests. Late syn-rift to rift narrowing (ca. 4 Myr duration): during the Langhian, the basin recorded several falls of relative sea level and bathymetry in the rift axis was progressively reduced. The former reliefs induced during the rift climax were quickly destroyed as evidenced by the drastic drop in sediment supply. Stratigraphic reconstruction indicates that the Central basin was restricted during lowstand period; meanwhile, open marine conditions prevailed to the north and south of the Suez rift. The Central basin, Zaafarana, and Morgan accommodation zones thus acted as a major divide between the Mediterranean Sea and the Red Sea. During Serravalian times, the Suez rift also recorded several disconnections between the Mediterranean and Red seas as evidenced by massive evaporites in major fault-controlled depocenters. The Suez rift was occasionally characterized by N–S paleogeographic gradient with restricted setting to the north and open marine setting to the south (Red Sea). Tectonic quiescence to latest syn-rift (ca. 7 Myr duration): the Tortonian was then characterized by the deposition of very thick salt series (>1000 m) which recorded a period of maximum restriction for the Suez rift. The basin was still subdivided into several sub-basins bounded by major faults. The basin with a N-S paleogeographic gradient was totally and permanently disconnected from the Mediterranean Sea and connected to open marine condition via the Red Sea. The Messinian was also characterized by a thick salt series, but the evaporite typology and sedimentary systems distribution suggest a more humid climate than during Tortonian times. Pre-salt to salt transition was not sharp and lasted for ca. 4 Myr (Langhian-Serravalian). It was initiated as the result of the combined effect of (1) climatic changes with aridization and low water input from the catchments and (2) rift dynamic induced by plate tectonic reorganization that controlled the interplay between sea level and accommodation zones constituting sills.

The introduction and spread of alien species is now recognized as one of the most significant modifiers of biodiversity. In the absence of their normal predators and parasites, alien crabs often establish high population densities and tend to compete fiercely with local fauna for food and shelter. A total of 73 species of brachyuran and crab-like anomuran decapods are known as alien species, of which 48 (65.8%) have become established. Three groups stand out with their high number of alien species: namely the Portunoidea (swimming crabs, such as Carcinus maenas), Grapsoidea (shore crabs, such as Hemigrapsus takanoi) and Majioidea (spider crabs, such as Pyromaia tuberculata). Canals, ballast water and hull fouling are the primary vectors/routes by which crabs are spread. Transfer of crabs with shellfish, combined with the live seafood trade, are also important. The Mediterranean Sea has the highest number of alien brachyuran species as many have invaded through the Suez Canal, making the Mediterranean the meeting place of Atlantic and Indo-West Pacific faunas. We used egg size as an indicator of life history strategies and a comparison of established alien species with a matched control group of crabs shows that mean egg size of alien crabs is smaller, but it shows wide variation. The Erythrean invaders from the Red Sea are a representative sample of aliens that shows the same pattern even though their transfer agent was a canal rather than shipping. Deliberate transfers to establish new fisheries has been successful in some cases (e.g., Paralithodes camtschaticus to the Barents Sea), but some species are still expanding their range and so their ultimate effects are unknown. The impact of most aliens remains uncertain, but recent work on Hemigrapsus sanguineus provides an excellent model of the kind of experimental field work that needs to be done. The current focus of attention on coastal aliens has resulted in the unfortunate agreement, at the international level, that ballast water can be dumped with impunity on the high seas, without any knowledge of its impact.

Introductions and transfers of species by ballast water in the Adriatic Sea