Abstract
Objective To establish a simple and convenient method and study the feasibility of using gold nanoparticle composites (ssDNA-AuNPs) for colorimetric detection of gamma radiation. Methods The composites ssDNA-AuNPs were prepared by applying the ssDNA to the surface of AuNPs, and then were irradiated with gamma ray with absorbed doses of 0, 5, 10, 20, and 30 Gy, respectively. Subsequently the color change in the solutions were observed simultaneously with absorption spectra being measured. The linear relationship between the ratio of A625/A521 in the absorption spectrum and the absorbed dose was established. Results With the increase of radiation dose, the color of the solutions changed from wine-red to blue-violet gradually, the ratio of A625/A521 in absorption spectra had excellent liner response for absorbed dose ranging from 0 to 30 Gy, and the linear equation was A625/A521=0.020 6+ 0.303 6 E(R2=0.991 5). Conclusions The ssDNA-AuNPs synthesized in this experiment can be successfully used for colorimetric detection of gamma absorbed dose, and a simple and convenient method for detection of gamma radiation has been newly established. Key words: Gold nanoparticle; γ-rays; Radiation dose; Colorimetric detection
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