Abstract
Calophyllum inophyllum has been known as a part of mangrove forest area. This species is distributed mostly in the coastal areas of Indonesia and Africa. It is rich of bioactive compounds and has been used as traditional medicine. The aim of this study was to investigate the effect of solvent types (acetone, ethanol, and methanol), solvent concentrations in water (50%, 80%, and 100%, v/v), and extraction temperatures (30°C, 45°C, and 60°C) on total phenolic content (TPC) from C. inophyllum leaves. The extraction was conducted with percolation method. This work employed a single replicate of one-factor-at-a-time experiment approach. The result shows that solvent type, solvent concentration in water, and extraction temperature have significant effect on the TPC value. On the other hand, they have less effect on the yield of crude extract obtained. The highest TPC (289,12 mg GAE/g of residue of C. inophyllum leaves powder) of crude extract was obtained with 80% methanol in water at the extraction temperature of 30°C at 48 h. Moreover, the antioxidant activity was also investigated by (2,2-diphenyl-1-picrylhydrazil) DPPH method. As the result, its IC50 value was 0.054 µg/mL.
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