Abstract
Objective To evaluated the relationship between the expression level of a forkhead transcription factor Foxo3a and the apoptosis of CD8+T lymphocytes in chronic hepatitis B patients from Tenth People's Hospital of Tongji University. Methods There were 42 cases of patients with chronic hepatitis B and 24 cases of normal control group. PBMC were derived from peripheral blood cells. The levels of intracellular cytokine of CD8+T cells were analyzed by flow cytometry. Cell apoptosis ratio was detected by Annexin V-FITC, Propidium Iodide (PI) and CD8 antibody three standard method. The expression of Foxo3a in CD8+T cells was analyzing by flow cytometry APC, FITC CD8 antibody and second antibodies, while the expression of Foxo3a in PBMC was detected by RT-PCR, and the protein level of Foxo3a was detected by western blot. The cytokines, cell apoptosis ratio and Foxo3a expression level in CD8+T cells were analyzed. Meanwhile, the apoptosis ratio of CD8+T, the relationships among the expression level of Foxo3a, the level of Foxo3a and CD8+T cell apoptosis were analyzed. Results The CD8+T apoptosis ratio[(21.18±2.48)%] in patients with chronic hepatitis B was higher than the normal control group[ (7.52±0.42) %] (t=18.34,P<0.05) . The expression of Foxo3a in patients with chronic hepatitis B was (0.55±0.99) and higher than the normal control group (0.14±0.03) , of which the difference had statistical significance (t=4.54,P<0.05). Multiple regression analysis showed that the linear regression relation between the expression of CD8+T apoptosis ratio and the level of Foxo3a (F=141.451,P<0.01) . Conclusions The increasing apoptosis and defective function of CD8+T lymphocytes in patients with chronic hepatitis B make the cell immune function to decrease, which is probably associated with the expression of Foxo3a. Key words: Hepatitis B, chronic; Cell apoptosis; Immune tolerance; Foxo3a; CD8+T cells
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