Abstract
Objective To explore the role of miR-98 in peripheral blood mononuclear cells (PBMC) in the pathogenesis and development of childhood asthma. Methods A total of 43 cases of asthmatic children and 30 cases of healthy controls were enrolled in the study.Peripheral blood mononuclear cells were isolated in both healthy subjects and asthmatic children in acute attack and remission stages.The expressions of miR-98 and interleukin-4(IL-4) and IL-13 mRNA were detected by real-time quantitative PCR. Results The miR-98 levels of asthmatic children in attack stage were significantly lower than those in remission stage and control group (P 0.05). Furthermore, a negative correlation was found between the expression of miR-98 and IL-5(r=-0.794, P<0.01) and between the expression of miR-98 and IL-13 mRNA (r=-0.804, P<0.01) in asthmatic children in attack stage.A positive correlation was also found between IL-4 and IL-13 mRNA in asthmatic children in attack stage (r=0.853, P<0.01). Conclusion The expression of miR-98 decreased in asthmatic children, and miR-98 might be involved in the pathogenesis and development of asthma. Key words: Asthma; MiR-98; Interleukin-4; Interleukin-13
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