The effects of the nitric oxide synthase inhibitors on the behaviour of small-platform-stressed mice in the plus-maze test

Abstract

Effects of the nitric oxide synthase (NOS) inhibitors 7-nitroindazole (7-NI), N G-nitro- l-arginine ( l-NOARG) and N G-nitro- l-arginine methyl ester ( l-NAME) on the behaviour of control and small-platform (SP)-stressed mice in the plus-maze test were studied. SP stress was induced by placing mice on SPs (3.5 cm diameter) surrounded by water for 24 h. This model contains several factors of stress like rapid eye movement (REM) sleep deprivation, isolation, immobilization and falling into the water. The plus-maze test was carried out with control and SP-stressed mice. SP stress induced an anxiolytic-like effect that was evidenced by increased percentage of time spent on the open arms of the plus-maze. The administration of NOS inhibitors 7-NI (20.0–120.0 mg/kg) and l-NOARG (20.0 and 40.0 mg/kg) induced an anxiolytic effect and the administration of l-NAME (20.0 and 40.0 mg/kg)—an anxiogenic effect in control mice. In SP-stressed mice, the effects of NOS inhibitors were changed. Contrary to control mice, 7-NI at a dose of 20.0 mg/kg induced an anxiogenic effect in SP-stressed mice and other doses of 7-NI, with exception of 80.0 mg/kg, as well as l-NOARG and l-NAME were without any effect. On the basis of these data, we can propose that SP stress induced changes in the function of l-arginine–NOS–NO pathways. It is also proposed that the behavioural effects of NOS inhibitors can be changed in stressed animals.