The Effects of S-Allyl Cysteıne on Inflammatory Cascade in Lipopolysaccharide Induced Rat Sepsis Model

Abstract

Lipopolysaccharide (LPS) is a main constituent of Gram-negative bacterial cell walls and is considered a leading cause of sepsis. S-allyl cysteine (SAC) is a water-soluble organosulfur component present in garlic which has a potent antioxidant and free radical scavenger activity. The purpose of this study is to examine the antioxidant and anti-inflammatory potential of SAC on endotoxin LPS-induced sepsis. Female Wistar albino rats were divided into 6 groups. LPS (5 mg/kg) was applied to rats in sepsis and treatment groups intraperitoneally. After 24 hours from LPS injection 50 mg/kg and 100 mg/kg SAC was orally administrated to treatment groups. Lung and liver 18F-fluoro-deoxy-D-glucose (18F-FDG) uptake was measured by 18FDG-PET scan. Serum levels of nuclear factor-kappa B (NF-κβ), tumor necrosis factor-alpha (TNF-α), matrix metalloproteinase-9 (MMP-9), plasma levels of interleukin-1β (IL-1β), IL-6 and tissue levels of oxidative stress markers catalase (CAT), superoxide dismutase (SOD), malondialdehyde (MDA) and nitric oxide (NO) were determined. As a result of the study, MDA and NO levels of sepsis group were significantly higher than treatment groups in lung tissue. SOD activities of sepsis group was determined to significantly lower in the liver and lung tissues than the groups which were treated with SAC. Likewise, it was concluded that serum MMP-9, TNF-α and NF-κβ levels of sepsis group was significantly higher compared to levels of treatment groups. It was determined that SAC administration reduced 18F-FDG uptake in septic rats. In conclusion, SAC was observed to diminish effects of the acute toxicity and oxidative stress formed with LPS.