The effects of quantified amounts of increased intermittent compressive forces for 30 and 60 days on the growth of limb bones in the rat.

Abstract

The purpose of this study was to subject groups of rats each to a specific 10% increase in body weight, to a maximum of a doubling of body weight, to study the effects of quantified, increased, intermittent, compressive forces on limb bone growth. Chronic centrifugation was employed. 21-day-old male Sprague-Dawley rats were used. After 30 and 60 days of centrifugation, the rats were killed. The humerus, radius, ulna, femur, and tibia were removed from each animal, cleared of all soft tissues, measured and weighed. The data were corrected for differences due to individual body weight by dividing bone lengths by the cube root of the body weight of the same animal. Tukey's 'Studentized' Multiple Range Test was performed to identify aggregations (sets) of force groups between which there are significant differences. The data suggest that the value of g, together with the amount of time spent at centrifugation, are both significant factors in the effects of increased intermittent, compressive forces, produced by simulated increases in body weight, on limb bone growth.