Abstract
Abstract Release of acetylcholine from rat isolated diaphragm preparations stimulated through the phrenic nerve was investigated in the presence of P-2-AM 0·01–100 mM. At 1 mM, the release of acetylcholine was increased by 157%, while higher concentrations resulted in a decrease. The findings indicate that the biphasic action of P-2-AM can be explained, at least in part, by variations in the quantal release of acetylcholine—an effect involving a presynaptic action.
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