Abstract

Humic waters (HW) are globally unique, deep underground, dark-brown waters containing humic acids, and they present numerous therapeutic activities including anti-inflammatory. In the present study, we use HW from source in Poland. Diabetes has become an epidemic and is a risk factor of cardiovascular diseases. Hyperglycemia in diabetes is responsible for damaging of the endothelium and increases inflammation on the surface of the vascular lining. The inflammatory process in diabetes is associated with the secretion of inflammatory cytokines by endothelial cells, e.g., tumor necrosis factor alpha (TNFα) and interleukin 6 (IL-6), and with the reduction of cell proliferation. In the study, we used cultures of endothelial cells (HUVEC line—human umbilical vein endothelial cells) with the addition 30 mM/L of glucose in the culture medium which imitated the conditions of uncontrolled diabetes. The addition of HW in the proper volume to the culture medium causes reduction of inflammation by significant decrease in inflammatory cytokines such as TNFα and IL-6 and also leads to enhancement of the cell proliferation. It appears that the adverse effects of hyperglycemia on vascular endothelial cells may be corrected by addition of humic water. The above promising results of in vitro tests provide an opportunity to the possible use of humic water in the supportive treatment of endothelial dysfunction disorders in diabetes. However, this issue requires further clinical research.

Highlights

  • Humic waters (HW) are globally unique, deep underground, dark-brown waters containing humic acids, found in only a few countries in Europe

  • The number of Human umbilical vein endothelial cells (HUVEC) was higher in group 2, and slightly lower in control group

  • The lowest number was observed in group 3, cultured under hyperglycemic conditions, while the number of cells in group 4 with glucose and humic water reached the level similar to the control group

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Summary

Introduction

Humic waters (HW) are globally unique, deep underground, dark-brown waters containing humic acids, found in only a few countries in Europe. Humic waters are a natural extract of humic substances (mainly humic and hymatomelanic acids) which give them a specific color. These acids are polymers consisting of an aromatic core of amino acids, sugars, peptides and other aliphatic components. They are decomposition products of organic matter of plant origin, formed during a process called humification (Latour et al 2015).

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