Abstract
Summary: Diploid strains of Coprinus lagopus grown on medium supplemented with griseofulvin (20 μg ml−1) produced fast-growing sectors of two types after 7 to 28 days incubation: these were diploid somatic recombinants and haploids. A dikaryon grown on the same medium broke down to give one haploid component only; resolution of this dikaryon and a near-isogenic diploid resulted in selection for sectors carrying a gene for resistance to griseofulvin. The use of this antibiotic is suggested as a tool for routine haploidization in C. lagopus.
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