Abstract
Abstract. The respiration rates and respiratory control ratios of isolated bean mitochondria have been measured following exposure to 0, 150, 300 and 900 J/m2 of far UV radiation (190–300 nm) from a mercury vapour light source with 90% total radiant intensity at 254 nm. Loss of respiratory control occurred at 150 J/m2 and inhibition of respiration was significant at the highest exposure dosage. The uptake of both 45Ca and 85Sr have been measured following a 10min incubation of isolated mitochondria with 2 mM cation. Significant decreases in cation accumulation were observed following exposure to 900 J/m2. The effect seemed to be associated with loss of active transport of the ions as a result of respiratory uncoupling or reduced electron transport. There was no significant effect of storage on respiration or ion transport nor was there any indirect effect of irradiated suspending medium on mitochondria.
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