Abstract
Covalent modification of arginine residues with phenylglyoxal and lysine residues with 4,4′-diisothiocyano-2,2′-disulphonic acid stilbene (DIDS) was carried out in preparations of rat cerebellar microsomes, as was the interaction of silver ions (silver nitrate) with cysteine residues. The effects of these amino acid-reactive agents on [ 3H]inositol 1,4,5-triphosphate (InsP 3) binding and InsP 3-induced calcium release were assessed. Both phenylglyoxal and DIDS inhibited [ 3H]InsP 3 binding and InsP 3-induced calcium release (IC 50 = 2.0 mM and 18 μM, respectively). Silver ions inhibited InsP 3-induced Ca 2+ release alone (IC ≈ 1 μM). These results suggest that arginine and lysine residues may be located at or close to the InsP 3 binding site of the InsP 3 receptor, while cysteine residues are important in channel opening.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
