The Effects of Allograft Combined with Ozone Therapy on Regeneration of Calvarial Defects in Rats

Abstract

Aim: Ozone accelerates wound healing and increases the blood supply to that in previous studies reported in the literature. In this study, we claim that ozone therapy can increase the bone regeneration of the combined used with bone allograft calvarial defects in rats. Material-Method: 12 male Wistar Rats were used in this study. Critical bone defects in the rat skulls were created by means of trephan bur (Mis Implant Tech, Shlomi, Israel) , opening a 5 mm diameter defect on the right side of the naso-frontal region of the skull. The rats were separated into 4 groups in random order;1-Control defect (n=6),2-Ozone application into the control defect (control+ ozone) group (n=6),3-Allograft group (n=6) 4-Ozone Combined with Allograft application (allograft + ozone) (n=6). The defects were administered at 80% density ozone for 14 days (Ozonytron, W&H, Burmoos, Austria). Rats sacrifice at 8. week Results: The osteoblast numbers were not significantly different in the control and control + ozone groups in the 8 th week .Allograft + ozone group, the osteoblast numbers increased at a statistically significant in the 8 th week when compared with the allograft group. Bone area measurements were statistically analyzed among the groups in the 8 th week, it was observed that the allograft + ozone group showed a significant increase in the bone area amount while no statistical significance was observed in the ozone-treated contol defects, and new bone formation was observed less in the control defects. Conclusions: The results of this study , allograft + ozone combination increases the osteoblast number and new bone area at an important level when compared with the allograft group alone.