The effects of acute ethanol challenge on amygdala protein and gene expression of mglu5 and pkc epsilon during ethanol withdrawal induced anxiety in rats

Abstract

Event Abstract Back to Event The effects of acute ethanol challenge on amygdala protein and gene expression of mglu5 and pkc epsilon during ethanol withdrawal induced anxiety in rats Jaya Kumar1, 2*, Hermizi Hapidin3, Yvonne Tee Get Bee3 and Zalina Ismail2 1 Universiti Kebangsaan Malaysia, Department of Physiology, UKM Medical Centre, Malaysia 2 Universiti Sains Malaysia, BRAINetwork Centre for Neurocognitive Sciences, Malaysia 3 Universiti Sains Malaysia, School of Health Sciences, Malaysia Cessation from prolonged and excessive ethanol intake can lead to hyperexcitability of glutamatergic neurotransmission in the amygdala, which induces an anxiety-like syndrome. Most alcoholics that suffer from such symptoms frequently depend on habitual drinking as self-medication to alleviate their symptoms. Metabotropic glutamate receptors 5 (mGlu5) and Protein Kinase C (PKC) epsilon have been related to ethanol consumption and anxiety-related behaviours. mGlu5 signalling has been associated with PKC activities and the epsilon isoform of PKC has been suggested as a crucial signalling target for modulation of ethanol consumption by mGlu5. This study explores the changes in mGlu5 and PKC epsilon at protein and gene-levels in the amygdala following acute ethanol challenge during EW induced anxiety in rats. Male Wistar rats were fed a modified liquid diet containing low fat cow milk, sucrose, and maltodextrin, with a gradual introduction of 2.4%, 4.8% and 7.2% ethanol for 20 days. Six hours into EW, the rats were intraperitoneally injected with normal saline and ethanol (2.5?g/kg, 20% v/v), and exposed to open field and elevated plus maze tests. Then, amygdala tissue was dissected from the rat brain for Western blot and gene expression studies. EW-induced anxiety was accompanied by a significant increment of mGlu5, total PKC epsilon and phosphorylated PKC epsilon protein levels and also of mRNA of mGlu5 (GRM5) in the amygdala. Acute administration of ethanol significantly attenuated EW-induced anxiety as well as an EW-induced increase in GRM5. The acute challenge of ethanol to EW rats had little effect on the phosphorylated and total protein levels of PKC epsilon in the amygdala. Our results show that amygdala PKC epsilon may not be directly involved in the development of anxiety following EW. Keywords: Amygdala, hyperexcitability, PKC, mGlu5, ethanol challenge Conference: 14th Meeting of the Asian-Pacific Society for Neurochemistry, Kuala Lumpur, Malaysia, 27 Aug - 30 Aug, 2016. Presentation Type: YIC05: Young Investigator Colloquium 5 Topic: 14th Meeting of the Asian-Pacific Society for Neurochemistry Citation: Kumar J, Hapidin H, Tee Get Bee Y and Ismail Z (2016). The effects of acute ethanol challenge on amygdala protein and gene expression of mglu5 and pkc epsilon during ethanol withdrawal induced anxiety in rats. Conference Abstract: 14th Meeting of the Asian-Pacific Society for Neurochemistry. doi: 10.3389/conf.fncel.2016.36.00069 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 04 Aug 2016; Published Online: 11 Aug 2016. * Correspondence: Dr. Jaya Kumar, Universiti Kebangsaan Malaysia, Department of Physiology, UKM Medical Centre, Cheras, Kuala Lumpur, Malaysia, jayakumar@ukm.edu.my Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Jaya Kumar Hermizi Hapidin Yvonne Tee Get Bee Zalina Ismail Google Jaya Kumar Hermizi Hapidin Yvonne Tee Get Bee Zalina Ismail Google Scholar Jaya Kumar Hermizi Hapidin Yvonne Tee Get Bee Zalina Ismail PubMed Jaya Kumar Hermizi Hapidin Yvonne Tee Get Bee Zalina Ismail Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.