The Effect of Temulawak (Curcuma xhantorrhiza) Dose on Tofu Dreg Plus Complete Feed Silage With Methane Relative Mitigation: In-Vitro

Abstract

Temulawak is an antimicrobial herb that can preserve the proteins in the feed from deamination and decay during the silage period. Tofu dregs are a feed ingredient that is difficult to preserve because they have high water content and protein. Temulawak powder may decrease deamination during the silage process because it has the ability as an antimicrobe and protein binder. Temulawak can reduce methane emissions by diminishing the number of protozoa that serve as hosts for methanogenic archaea. Temulawak with a harvest age of 3 months after planting, then dried at 65°C to constant weight, grounded, and tested on mix ration silage at doses of 0%, 0.25%, 0.5%, 0.75 %, and 1% from feed dry matter (DM). The experiment was carried out four times using a complete random design and in vitro silage was evaluated in the following 14 days. The results indicated that adding temulawak would reduce ammonia production with p<0.01, significantly increase the crude protein content of silage P<0.05 and decrease rumen ammonia P<0.01. The treatment increased total volatile fatty acid (VFA) production P<0.01), and reduced methane level P<0.01. The methane relative to dry matter (DM), organic matter, and total gas revealed a significant decrease with P<0.05. The 0.25% treatment level had the lowest total methane concentration and relative. Concluded that the optimal dose of temulawak addition to complete feed plus tofu dreg silage is 0.25% from DM.