Abstract
Periodontitis is a major risk factor in the oral cavity in chronic diabetes mellitus. The level of IL-1β as inflammatory cytokine increased in patients with type 2 diabetes and periodontitis, while IL-10 is an anti-inflammatory cytokine, may mediate periodontitis in diabetes. Stichopus hermanii (SH) have been known to have anti-inflammation property. Hyperbaric Oxygen Therapy (HBOT) thas been used as adjuvant therapy in diabetes wound healing. Purpose: To examine the effect of combination SH and HBOT to the expression of inflammatory response of IL-1β and IL-10 on diabetic periodontitis rats. Methods :Thirty male Wistar rats were divided to normal (K-0), diabetic-periodontitis (K-1), and treatment group of SH (K-2), HBOT (K-3), and SH-HBOT (K-4).Experimental diabetic was induced by single dose 65 mg/kg of BW intraperitoneal. The Sticopus hermanii gel 3% by topical application in sulcus gingiva and HBOT 2,4 ATA 3x30 minutes interval 5 minute for 7 days. After 52 days the animals were decapitated and the expression of IL-1β and IL-10 were examined by immunohistochemistry. Data were analyzed with ANOVA and LSD. Results : The expression of IL-1β were raised in K-1 group (10.68±1.50) (p< 0.05), while IL-10 were not raised (8.00 ±1.95) compare to normal group (7.25 ± 0.85) (p>0.05). Treatment with SH in K-2 group resulted in decreased of IL-1β expression (10.00± 1.09) while the expression of IL-10 were raised (8.33 ± 2.60) compare to K-1 (7.25 ± 0.85) (p<0.05). Treatment with HBOT in K-3 group resulted in decreased of IL-10 expression (8.333±0,81) while the expression of IL- 10 were raised (10.67±1.03) compare to K-1 (p<0.05). Treatment with SH-HBOT in K-4 group showed the most decreasing expression of IL-1β (6.50±2.44) and increasing expression of IL-10 (14.83±1.47) compare to K-1 (p<0.05). Conclusions : Combination of SH-HBOT decreased the expression of IL-1β and increased the expression of IL-10 on diabetic periodontitis diabetes rats
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